Hematoxylin & Eosin staining
Hematoxylin stains nuclei blue / purple. Eosin stains the cytoplasm pink. Several protocol variants exist for H & E staining. The most basic variant is described below.
For paraffin sections wash with Xylene for 5 minutes, followed by 100% ethanol for 2 minutes, 90% ethanol for 2 minutes, 70% ethanol for 2 minutes, and finally deionised water for 2 minutes. Go to step 1 below.
For cryo-sections, air dry slides then wash / hydrate slides in deionised water for 2 minutes. (May need to repeat the 2 minute water wash). Go to step 1 below.
1. Hematoxylin for 5 – 10 minutes
2. Wash with deionised water changing water periodically
3. Eosin for 0.5 – 2 minutes
4. Wash with 90% ethanol
5. Wash with 100% ethanol
6. Wash with Xylene